Description
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Bromazolam: Forensic Profile and Research Overview
Chemical Background
Bromazolam is a designer benzodiazepine, structurally related to alprazolam, but with a bromine atom replacing the chlorine atom found in its pharmaceutical counterpart. It was first synthesized in the 1970s during medicinal drug development but was never approved for therapeutic use. Despite its lack of medical authorization, bromazolam has emerged in the illicit drug market, particularly in counterfeit benzodiazepine tablets and powders.
Its chemical formula is C17H13BrN4, and it functions as a central nervous system depressant, binding to GABA-A receptors to produce sedative, anxiolytic, and muscle-relaxant effects. These pharmacological properties make it attractive for recreational misuse, but also pose significant risks due to its unpredictable potency and lack of clinical oversight. Buy Bromazolam
Forensic Detection and Analytical Methods
In forensic toxicology, bromazolam is typically detected using liquid chromatography–tandem mass spectrometry (LC–MS/MS) or high-resolution accurate mass spectrometry (HRAM-MS). These methods allow for precise quantification in biological matrices such as blood, urine, and postmortem samples.
A study published in the Journal of Analytical Toxicology reported bromazolam in 98 impaired driving cases between 2021 and 2023. Blood concentrations ranged from 4.2 to 990 ng/mL, with a mean of 125 ng/mL. These findings underscore its growing prevalence and the need for routine screening in forensic casework.
Prevalence and Public Health Concerns
Bromazolam has seen a surge in detection across the United States, particularly in forensic toxicology labs. According to the Center for Forensic Science Research and Education (CFSRE), bromazolam was identified in over 250 toxicology cases, including both antemortem and postmortem investigations.
Its rise is closely tied to its co-detection with fentanyl, a potent synthetic opioid. In recent analyses, over 75% of bromazolam-positive samples also contained fentanyl, suggesting a dangerous trend of polydrug use. This combination significantly increases the risk of respiratory depression, overdose, and death, making bromazolam a priority in forensic and public health surveillance.
Behavioral and Impairment Indicators
In impaired driving investigations, individuals testing positive for bromazolam exhibited behaviors consistent with central nervous system depression. These included:
- Slurred speech
- Incoordination
- Lethargy
- Errors in Standardized Field Sobriety Tests
- Erratic driving patterns
Such symptoms mirror those of other benzodiazepines but may be more pronounced due to bromazolam’s unregulated potency and frequent combination with other substances.
Postmortem Findings
A separate forensic study documented 96 postmortem cases involving bromazolam between 2021 and 2022. Blood concentrations varied widely, and co-findings often included opioids, stimulants, and other benzodiazepines. These cases highlight bromazolam’s role in polypharmacy fatalities, where its presence complicates cause-of-death determinations and toxicological interpretations.
Challenges in Forensic Interpretation
Several factors complicate the forensic analysis of bromazolam:
- Lack of standardized reference materials
- Limited pharmacokinetic data
- Rapid emergence of analogs
- Variable purity in street samples
These challenges necessitate ongoing research and collaboration among forensic laboratories to improve detection methods, interpretive guidelines, and public health responses.
Regulatory Status and Legal Implications
Bromazolam is not approved for medical use in most jurisdictions and is often classified under emerging psychoactive substances (EPS). Its legal status varies by country and region, with some areas moving to schedule it due to its abuse potential and public health impact.
In forensic contexts, its presence may indicate illicit drug use, counterfeit medication distribution, or intentional impairment, depending on the case scenario. As such, it plays a critical role in criminal investigations, coronial inquiries, and toxicological surveillance. Buy Bromazolam online.
Research Outlook
The forensic and scientific community continues to monitor bromazolam due to its:
- Increasing prevalence in toxicology reports
- Association with fatal overdoses
- Role in impaired driving and behavioral incidents
- Emergence in counterfeit pharmaceuticals
Future research priorities include:
- Establishing dose-response relationships
- Understanding metabolic pathways
- Developing rapid field detection tools
- Evaluating long-term health effects
Conclusion
Bromazolam represents a significant challenge in forensic science due to its potency, prevalence, and unpredictability. While not intended for human consumption, its detection in forensic investigations provides critical insights into drug trends, public safety risks, and toxicological complexities. Continued vigilance, research, and analytical innovation are essential to address the evolving landscape of designer benzodiazepines.
Bromazolam
Cat. No. B1280215
CAS RN: 71368-80-4
Formula: C17H13BrN4
Molecular Weight: 353.2 g/mol
InChI Key: KCEIOBKDDQAYCM-UHFFFAOYSA-N
IUPAC Name: 8-bromo-1-methyl-6-phenyl-4H-triazolo, benzodiazepine
InChI InChI=1S/C17H13BrN4/c1-11-20-21-16-10-19-17(12-5-3-2-4-6-12)14-9-13(18)7-8-15(14)22(11)16/h2-9H,10H2,1H3
SMILES CC1=NN=C2N1C3=C(C=C(C=C3)Br)C(=NC2)C4=CC=CC=C4
Canonical SMILES CC1=NN=C2N1C3=C(C=C(C=C3)Br)C(=NC2)C4=CC=CC=C4
Uses of Bromazolam
Bromazolam is the Bromo instead of chloro analog of alprazolam and has sedative and anxiolytic effects and other benzodiazepines. Bromazolam is commonly reported in combination with other drugs, including the opioid fentanyl.
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Bromazolam is a non-subtype selective agonist at the benzodiazepine site of GABAA receptors, with a binding affinity of 2.81nM at the α1 subtype, 0.69nM at α2 and 0.62nM at α5.
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